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type 1 bovine collagen solution  (Advanced Biomatrix Inc)

 
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    Advanced Biomatrix Inc type 1 bovine collagen solution
    Type 1 Bovine Collagen Solution, supplied by Advanced Biomatrix Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bovine+collagen%2C+type+1/collagen/pmc12989087-37-0-8
    Average 86 stars, based on 1 article reviews
    type 1 bovine collagen solution - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Recombinant:

    Article Title: Direct Arp2/3-vinculin binding is required for pseudopod extension, but only on compliant substrates and in 3D
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse anti-mDia1/DIAPH1 BD Biosciences Cat# BD 610848; RRID: AB_398167 mouse anti-ARP3 (A-1) Santa Cruz Cat# sc-48344; RRID: AB_626700 mouse anti-ARP3, (B-1) Santa Cruz Cat# sc-374200; RRID: AB_10990131 rabbit anti-Cofilin-1 D3F9 Cell Signaling Technology Cat# 5175; RRID: AB_10622000 rabbit anti-alpha Actinin Abcam Cat# ab175944 mouse anti-FAK (D-1) Santa Cruz Cat# sc-271126; RRID: AB_10614323 mouse anti-FMNL1, (C-5) Santa Cruz Cat# sc-390023 mouse anti-FMNL2 Abcam Cat# ab57963; RRID: AB_941625 rabbit anti-DIAPH3 ProteinTech Cat# 14342-1-AP; RRID: AB_2092930 rabbit anti-INF2 Bethyl Laboratories Cat# A303-428A; RRID: AB_10952400 mouse anti-FHOD1, (D-6) Santa Cruz Cat# sc-365437; RRID: AB_10843854 mouse anti-VASP BD Biosciences Cat# BD 610447 mouse anti-Mena (21) Santa Cruz Cat# sc-135988; RRID: AB_2098633 rabbit anti-Cortactin, [EP1922Y] Abcam Cat# ab81208; RRID: AB_1640383 mouse anti-CapZ-β, (52) Santa Cruz Cat# sc-136502; RRID: AB_10610091 rabbit anti-WAVE-2, (D2C8) XP Cell Signaling Technology Cat# 3659 rabbit anti-N-WASP (30D10) Cell Signaling Technology Cat# 4848 mouse anti-vinculin, (7F9) Santa Cruz Cat# sc-73614; RRID: AB_2941767 mouse anti-vinculin Sigma-Aldrich Cat# V9264; RRID: AB_10603627 mouse anti-Kindlin-2, clone 3A3 Millipore Cat# MAB2617; RRID: AB_10631873 rabbit anti-p34-Arc/ARPC2 Millipore Cat# 07-227 mNeonGreen-Trap Magnetic Agarose beads Chromotek Cat# ntma-20; RRID: AB_2827594 Chemicals, peptides, and recombinant proteins blasticidine S HCl Thermo R21001 Polyethylenimine Sigma 408727 Platinum SuperFi II PCR Master Mix Thermo Cat # 12368010 AF488-conjugated Phalloidin Thermo A12379 AF568-conjugated Phalloidin Thermo A12380 Bovine collagen, Type 1 Advanced Biomatrix Cat# 5133 rat-tail collagen Corning Cat# 354249 CK-666 Millipore Cat# 182515 purified Arp2/3 complex Cytoskeleton RP-01-PA QGel 920 CHT QGel 920 20% Paraformaldehyde Electron Microscopy Sciences 15713 Critical commercial assays Q5 site-directed PCR mutagenesis kit NEB E0554S DNeasy Blood and Tissue kit Qiagen Cat # 69504 QIAquick Gel Extraction Kit Qiagen Cat # 28704 Duolink® In Situ Detection Red kit Sigma DUO92008 Experimental models: Cell lines U2OS Gifted by Dick McIntosh (University of Colorado) RPE-1 ATCC CRL-4000; RRID: CVCL_4388 (Continued on next page) iScience 28, 112623, June 20, 2025 e1 .. Cell lines and culture conditions Human Osteosarcoma (U2OS; female) and human retinal pigment epithelial (RPE-1; female) cells were cultured in DMEM or RPMI media, respectively supplemented with 10% fetal bovine serum (Sigma; F0926-500ML) and maintained in a humidified incubator at 37◦C and 5% CO2.

    Polymerase Chain Reaction:

    Article Title: Direct Arp2/3-vinculin binding is required for pseudopod extension, but only on compliant substrates and in 3D
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse anti-mDia1/DIAPH1 BD Biosciences Cat# BD 610848; RRID: AB_398167 mouse anti-ARP3 (A-1) Santa Cruz Cat# sc-48344; RRID: AB_626700 mouse anti-ARP3, (B-1) Santa Cruz Cat# sc-374200; RRID: AB_10990131 rabbit anti-Cofilin-1 D3F9 Cell Signaling Technology Cat# 5175; RRID: AB_10622000 rabbit anti-alpha Actinin Abcam Cat# ab175944 mouse anti-FAK (D-1) Santa Cruz Cat# sc-271126; RRID: AB_10614323 mouse anti-FMNL1, (C-5) Santa Cruz Cat# sc-390023 mouse anti-FMNL2 Abcam Cat# ab57963; RRID: AB_941625 rabbit anti-DIAPH3 ProteinTech Cat# 14342-1-AP; RRID: AB_2092930 rabbit anti-INF2 Bethyl Laboratories Cat# A303-428A; RRID: AB_10952400 mouse anti-FHOD1, (D-6) Santa Cruz Cat# sc-365437; RRID: AB_10843854 mouse anti-VASP BD Biosciences Cat# BD 610447 mouse anti-Mena (21) Santa Cruz Cat# sc-135988; RRID: AB_2098633 rabbit anti-Cortactin, [EP1922Y] Abcam Cat# ab81208; RRID: AB_1640383 mouse anti-CapZ-β, (52) Santa Cruz Cat# sc-136502; RRID: AB_10610091 rabbit anti-WAVE-2, (D2C8) XP Cell Signaling Technology Cat# 3659 rabbit anti-N-WASP (30D10) Cell Signaling Technology Cat# 4848 mouse anti-vinculin, (7F9) Santa Cruz Cat# sc-73614; RRID: AB_2941767 mouse anti-vinculin Sigma-Aldrich Cat# V9264; RRID: AB_10603627 mouse anti-Kindlin-2, clone 3A3 Millipore Cat# MAB2617; RRID: AB_10631873 rabbit anti-p34-Arc/ARPC2 Millipore Cat# 07-227 mNeonGreen-Trap Magnetic Agarose beads Chromotek Cat# ntma-20; RRID: AB_2827594 Chemicals, peptides, and recombinant proteins blasticidine S HCl Thermo R21001 Polyethylenimine Sigma 408727 Platinum SuperFi II PCR Master Mix Thermo Cat # 12368010 AF488-conjugated Phalloidin Thermo A12379 AF568-conjugated Phalloidin Thermo A12380 Bovine collagen, Type 1 Advanced Biomatrix Cat# 5133 rat-tail collagen Corning Cat# 354249 CK-666 Millipore Cat# 182515 purified Arp2/3 complex Cytoskeleton RP-01-PA QGel 920 CHT QGel 920 20% Paraformaldehyde Electron Microscopy Sciences 15713 Critical commercial assays Q5 site-directed PCR mutagenesis kit NEB E0554S DNeasy Blood and Tissue kit Qiagen Cat # 69504 QIAquick Gel Extraction Kit Qiagen Cat # 28704 Duolink® In Situ Detection Red kit Sigma DUO92008 Experimental models: Cell lines U2OS Gifted by Dick McIntosh (University of Colorado) RPE-1 ATCC CRL-4000; RRID: CVCL_4388 (Continued on next page) iScience 28, 112623, June 20, 2025 e1 .. Cell lines and culture conditions Human Osteosarcoma (U2OS; female) and human retinal pigment epithelial (RPE-1; female) cells were cultured in DMEM or RPMI media, respectively supplemented with 10% fetal bovine serum (Sigma; F0926-500ML) and maintained in a humidified incubator at 37◦C and 5% CO2.

    Purification:

    Article Title: Direct Arp2/3-vinculin binding is required for pseudopod extension, but only on compliant substrates and in 3D
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse anti-mDia1/DIAPH1 BD Biosciences Cat# BD 610848; RRID: AB_398167 mouse anti-ARP3 (A-1) Santa Cruz Cat# sc-48344; RRID: AB_626700 mouse anti-ARP3, (B-1) Santa Cruz Cat# sc-374200; RRID: AB_10990131 rabbit anti-Cofilin-1 D3F9 Cell Signaling Technology Cat# 5175; RRID: AB_10622000 rabbit anti-alpha Actinin Abcam Cat# ab175944 mouse anti-FAK (D-1) Santa Cruz Cat# sc-271126; RRID: AB_10614323 mouse anti-FMNL1, (C-5) Santa Cruz Cat# sc-390023 mouse anti-FMNL2 Abcam Cat# ab57963; RRID: AB_941625 rabbit anti-DIAPH3 ProteinTech Cat# 14342-1-AP; RRID: AB_2092930 rabbit anti-INF2 Bethyl Laboratories Cat# A303-428A; RRID: AB_10952400 mouse anti-FHOD1, (D-6) Santa Cruz Cat# sc-365437; RRID: AB_10843854 mouse anti-VASP BD Biosciences Cat# BD 610447 mouse anti-Mena (21) Santa Cruz Cat# sc-135988; RRID: AB_2098633 rabbit anti-Cortactin, [EP1922Y] Abcam Cat# ab81208; RRID: AB_1640383 mouse anti-CapZ-β, (52) Santa Cruz Cat# sc-136502; RRID: AB_10610091 rabbit anti-WAVE-2, (D2C8) XP Cell Signaling Technology Cat# 3659 rabbit anti-N-WASP (30D10) Cell Signaling Technology Cat# 4848 mouse anti-vinculin, (7F9) Santa Cruz Cat# sc-73614; RRID: AB_2941767 mouse anti-vinculin Sigma-Aldrich Cat# V9264; RRID: AB_10603627 mouse anti-Kindlin-2, clone 3A3 Millipore Cat# MAB2617; RRID: AB_10631873 rabbit anti-p34-Arc/ARPC2 Millipore Cat# 07-227 mNeonGreen-Trap Magnetic Agarose beads Chromotek Cat# ntma-20; RRID: AB_2827594 Chemicals, peptides, and recombinant proteins blasticidine S HCl Thermo R21001 Polyethylenimine Sigma 408727 Platinum SuperFi II PCR Master Mix Thermo Cat # 12368010 AF488-conjugated Phalloidin Thermo A12379 AF568-conjugated Phalloidin Thermo A12380 Bovine collagen, Type 1 Advanced Biomatrix Cat# 5133 rat-tail collagen Corning Cat# 354249 CK-666 Millipore Cat# 182515 purified Arp2/3 complex Cytoskeleton RP-01-PA QGel 920 CHT QGel 920 20% Paraformaldehyde Electron Microscopy Sciences 15713 Critical commercial assays Q5 site-directed PCR mutagenesis kit NEB E0554S DNeasy Blood and Tissue kit Qiagen Cat # 69504 QIAquick Gel Extraction Kit Qiagen Cat # 28704 Duolink® In Situ Detection Red kit Sigma DUO92008 Experimental models: Cell lines U2OS Gifted by Dick McIntosh (University of Colorado) RPE-1 ATCC CRL-4000; RRID: CVCL_4388 (Continued on next page) iScience 28, 112623, June 20, 2025 e1 .. Cell lines and culture conditions Human Osteosarcoma (U2OS; female) and human retinal pigment epithelial (RPE-1; female) cells were cultured in DMEM or RPMI media, respectively supplemented with 10% fetal bovine serum (Sigma; F0926-500ML) and maintained in a humidified incubator at 37◦C and 5% CO2.

    Electron Microscopy:

    Article Title: Direct Arp2/3-vinculin binding is required for pseudopod extension, but only on compliant substrates and in 3D
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse anti-mDia1/DIAPH1 BD Biosciences Cat# BD 610848; RRID: AB_398167 mouse anti-ARP3 (A-1) Santa Cruz Cat# sc-48344; RRID: AB_626700 mouse anti-ARP3, (B-1) Santa Cruz Cat# sc-374200; RRID: AB_10990131 rabbit anti-Cofilin-1 D3F9 Cell Signaling Technology Cat# 5175; RRID: AB_10622000 rabbit anti-alpha Actinin Abcam Cat# ab175944 mouse anti-FAK (D-1) Santa Cruz Cat# sc-271126; RRID: AB_10614323 mouse anti-FMNL1, (C-5) Santa Cruz Cat# sc-390023 mouse anti-FMNL2 Abcam Cat# ab57963; RRID: AB_941625 rabbit anti-DIAPH3 ProteinTech Cat# 14342-1-AP; RRID: AB_2092930 rabbit anti-INF2 Bethyl Laboratories Cat# A303-428A; RRID: AB_10952400 mouse anti-FHOD1, (D-6) Santa Cruz Cat# sc-365437; RRID: AB_10843854 mouse anti-VASP BD Biosciences Cat# BD 610447 mouse anti-Mena (21) Santa Cruz Cat# sc-135988; RRID: AB_2098633 rabbit anti-Cortactin, [EP1922Y] Abcam Cat# ab81208; RRID: AB_1640383 mouse anti-CapZ-β, (52) Santa Cruz Cat# sc-136502; RRID: AB_10610091 rabbit anti-WAVE-2, (D2C8) XP Cell Signaling Technology Cat# 3659 rabbit anti-N-WASP (30D10) Cell Signaling Technology Cat# 4848 mouse anti-vinculin, (7F9) Santa Cruz Cat# sc-73614; RRID: AB_2941767 mouse anti-vinculin Sigma-Aldrich Cat# V9264; RRID: AB_10603627 mouse anti-Kindlin-2, clone 3A3 Millipore Cat# MAB2617; RRID: AB_10631873 rabbit anti-p34-Arc/ARPC2 Millipore Cat# 07-227 mNeonGreen-Trap Magnetic Agarose beads Chromotek Cat# ntma-20; RRID: AB_2827594 Chemicals, peptides, and recombinant proteins blasticidine S HCl Thermo R21001 Polyethylenimine Sigma 408727 Platinum SuperFi II PCR Master Mix Thermo Cat # 12368010 AF488-conjugated Phalloidin Thermo A12379 AF568-conjugated Phalloidin Thermo A12380 Bovine collagen, Type 1 Advanced Biomatrix Cat# 5133 rat-tail collagen Corning Cat# 354249 CK-666 Millipore Cat# 182515 purified Arp2/3 complex Cytoskeleton RP-01-PA QGel 920 CHT QGel 920 20% Paraformaldehyde Electron Microscopy Sciences 15713 Critical commercial assays Q5 site-directed PCR mutagenesis kit NEB E0554S DNeasy Blood and Tissue kit Qiagen Cat # 69504 QIAquick Gel Extraction Kit Qiagen Cat # 28704 Duolink® In Situ Detection Red kit Sigma DUO92008 Experimental models: Cell lines U2OS Gifted by Dick McIntosh (University of Colorado) RPE-1 ATCC CRL-4000; RRID: CVCL_4388 (Continued on next page) iScience 28, 112623, June 20, 2025 e1 .. Cell lines and culture conditions Human Osteosarcoma (U2OS; female) and human retinal pigment epithelial (RPE-1; female) cells were cultured in DMEM or RPMI media, respectively supplemented with 10% fetal bovine serum (Sigma; F0926-500ML) and maintained in a humidified incubator at 37◦C and 5% CO2.

    Mutagenesis:

    Article Title: Direct Arp2/3-vinculin binding is required for pseudopod extension, but only on compliant substrates and in 3D
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse anti-mDia1/DIAPH1 BD Biosciences Cat# BD 610848; RRID: AB_398167 mouse anti-ARP3 (A-1) Santa Cruz Cat# sc-48344; RRID: AB_626700 mouse anti-ARP3, (B-1) Santa Cruz Cat# sc-374200; RRID: AB_10990131 rabbit anti-Cofilin-1 D3F9 Cell Signaling Technology Cat# 5175; RRID: AB_10622000 rabbit anti-alpha Actinin Abcam Cat# ab175944 mouse anti-FAK (D-1) Santa Cruz Cat# sc-271126; RRID: AB_10614323 mouse anti-FMNL1, (C-5) Santa Cruz Cat# sc-390023 mouse anti-FMNL2 Abcam Cat# ab57963; RRID: AB_941625 rabbit anti-DIAPH3 ProteinTech Cat# 14342-1-AP; RRID: AB_2092930 rabbit anti-INF2 Bethyl Laboratories Cat# A303-428A; RRID: AB_10952400 mouse anti-FHOD1, (D-6) Santa Cruz Cat# sc-365437; RRID: AB_10843854 mouse anti-VASP BD Biosciences Cat# BD 610447 mouse anti-Mena (21) Santa Cruz Cat# sc-135988; RRID: AB_2098633 rabbit anti-Cortactin, [EP1922Y] Abcam Cat# ab81208; RRID: AB_1640383 mouse anti-CapZ-β, (52) Santa Cruz Cat# sc-136502; RRID: AB_10610091 rabbit anti-WAVE-2, (D2C8) XP Cell Signaling Technology Cat# 3659 rabbit anti-N-WASP (30D10) Cell Signaling Technology Cat# 4848 mouse anti-vinculin, (7F9) Santa Cruz Cat# sc-73614; RRID: AB_2941767 mouse anti-vinculin Sigma-Aldrich Cat# V9264; RRID: AB_10603627 mouse anti-Kindlin-2, clone 3A3 Millipore Cat# MAB2617; RRID: AB_10631873 rabbit anti-p34-Arc/ARPC2 Millipore Cat# 07-227 mNeonGreen-Trap Magnetic Agarose beads Chromotek Cat# ntma-20; RRID: AB_2827594 Chemicals, peptides, and recombinant proteins blasticidine S HCl Thermo R21001 Polyethylenimine Sigma 408727 Platinum SuperFi II PCR Master Mix Thermo Cat # 12368010 AF488-conjugated Phalloidin Thermo A12379 AF568-conjugated Phalloidin Thermo A12380 Bovine collagen, Type 1 Advanced Biomatrix Cat# 5133 rat-tail collagen Corning Cat# 354249 CK-666 Millipore Cat# 182515 purified Arp2/3 complex Cytoskeleton RP-01-PA QGel 920 CHT QGel 920 20% Paraformaldehyde Electron Microscopy Sciences 15713 Critical commercial assays Q5 site-directed PCR mutagenesis kit NEB E0554S DNeasy Blood and Tissue kit Qiagen Cat # 69504 QIAquick Gel Extraction Kit Qiagen Cat # 28704 Duolink® In Situ Detection Red kit Sigma DUO92008 Experimental models: Cell lines U2OS Gifted by Dick McIntosh (University of Colorado) RPE-1 ATCC CRL-4000; RRID: CVCL_4388 (Continued on next page) iScience 28, 112623, June 20, 2025 e1 .. Cell lines and culture conditions Human Osteosarcoma (U2OS; female) and human retinal pigment epithelial (RPE-1; female) cells were cultured in DMEM or RPMI media, respectively supplemented with 10% fetal bovine serum (Sigma; F0926-500ML) and maintained in a humidified incubator at 37◦C and 5% CO2.

    Gel Extraction:

    Article Title: Direct Arp2/3-vinculin binding is required for pseudopod extension, but only on compliant substrates and in 3D
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse anti-mDia1/DIAPH1 BD Biosciences Cat# BD 610848; RRID: AB_398167 mouse anti-ARP3 (A-1) Santa Cruz Cat# sc-48344; RRID: AB_626700 mouse anti-ARP3, (B-1) Santa Cruz Cat# sc-374200; RRID: AB_10990131 rabbit anti-Cofilin-1 D3F9 Cell Signaling Technology Cat# 5175; RRID: AB_10622000 rabbit anti-alpha Actinin Abcam Cat# ab175944 mouse anti-FAK (D-1) Santa Cruz Cat# sc-271126; RRID: AB_10614323 mouse anti-FMNL1, (C-5) Santa Cruz Cat# sc-390023 mouse anti-FMNL2 Abcam Cat# ab57963; RRID: AB_941625 rabbit anti-DIAPH3 ProteinTech Cat# 14342-1-AP; RRID: AB_2092930 rabbit anti-INF2 Bethyl Laboratories Cat# A303-428A; RRID: AB_10952400 mouse anti-FHOD1, (D-6) Santa Cruz Cat# sc-365437; RRID: AB_10843854 mouse anti-VASP BD Biosciences Cat# BD 610447 mouse anti-Mena (21) Santa Cruz Cat# sc-135988; RRID: AB_2098633 rabbit anti-Cortactin, [EP1922Y] Abcam Cat# ab81208; RRID: AB_1640383 mouse anti-CapZ-β, (52) Santa Cruz Cat# sc-136502; RRID: AB_10610091 rabbit anti-WAVE-2, (D2C8) XP Cell Signaling Technology Cat# 3659 rabbit anti-N-WASP (30D10) Cell Signaling Technology Cat# 4848 mouse anti-vinculin, (7F9) Santa Cruz Cat# sc-73614; RRID: AB_2941767 mouse anti-vinculin Sigma-Aldrich Cat# V9264; RRID: AB_10603627 mouse anti-Kindlin-2, clone 3A3 Millipore Cat# MAB2617; RRID: AB_10631873 rabbit anti-p34-Arc/ARPC2 Millipore Cat# 07-227 mNeonGreen-Trap Magnetic Agarose beads Chromotek Cat# ntma-20; RRID: AB_2827594 Chemicals, peptides, and recombinant proteins blasticidine S HCl Thermo R21001 Polyethylenimine Sigma 408727 Platinum SuperFi II PCR Master Mix Thermo Cat # 12368010 AF488-conjugated Phalloidin Thermo A12379 AF568-conjugated Phalloidin Thermo A12380 Bovine collagen, Type 1 Advanced Biomatrix Cat# 5133 rat-tail collagen Corning Cat# 354249 CK-666 Millipore Cat# 182515 purified Arp2/3 complex Cytoskeleton RP-01-PA QGel 920 CHT QGel 920 20% Paraformaldehyde Electron Microscopy Sciences 15713 Critical commercial assays Q5 site-directed PCR mutagenesis kit NEB E0554S DNeasy Blood and Tissue kit Qiagen Cat # 69504 QIAquick Gel Extraction Kit Qiagen Cat # 28704 Duolink® In Situ Detection Red kit Sigma DUO92008 Experimental models: Cell lines U2OS Gifted by Dick McIntosh (University of Colorado) RPE-1 ATCC CRL-4000; RRID: CVCL_4388 (Continued on next page) iScience 28, 112623, June 20, 2025 e1 .. Cell lines and culture conditions Human Osteosarcoma (U2OS; female) and human retinal pigment epithelial (RPE-1; female) cells were cultured in DMEM or RPMI media, respectively supplemented with 10% fetal bovine serum (Sigma; F0926-500ML) and maintained in a humidified incubator at 37◦C and 5% CO2.

    In Situ:

    Article Title: Direct Arp2/3-vinculin binding is required for pseudopod extension, but only on compliant substrates and in 3D
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse anti-mDia1/DIAPH1 BD Biosciences Cat# BD 610848; RRID: AB_398167 mouse anti-ARP3 (A-1) Santa Cruz Cat# sc-48344; RRID: AB_626700 mouse anti-ARP3, (B-1) Santa Cruz Cat# sc-374200; RRID: AB_10990131 rabbit anti-Cofilin-1 D3F9 Cell Signaling Technology Cat# 5175; RRID: AB_10622000 rabbit anti-alpha Actinin Abcam Cat# ab175944 mouse anti-FAK (D-1) Santa Cruz Cat# sc-271126; RRID: AB_10614323 mouse anti-FMNL1, (C-5) Santa Cruz Cat# sc-390023 mouse anti-FMNL2 Abcam Cat# ab57963; RRID: AB_941625 rabbit anti-DIAPH3 ProteinTech Cat# 14342-1-AP; RRID: AB_2092930 rabbit anti-INF2 Bethyl Laboratories Cat# A303-428A; RRID: AB_10952400 mouse anti-FHOD1, (D-6) Santa Cruz Cat# sc-365437; RRID: AB_10843854 mouse anti-VASP BD Biosciences Cat# BD 610447 mouse anti-Mena (21) Santa Cruz Cat# sc-135988; RRID: AB_2098633 rabbit anti-Cortactin, [EP1922Y] Abcam Cat# ab81208; RRID: AB_1640383 mouse anti-CapZ-β, (52) Santa Cruz Cat# sc-136502; RRID: AB_10610091 rabbit anti-WAVE-2, (D2C8) XP Cell Signaling Technology Cat# 3659 rabbit anti-N-WASP (30D10) Cell Signaling Technology Cat# 4848 mouse anti-vinculin, (7F9) Santa Cruz Cat# sc-73614; RRID: AB_2941767 mouse anti-vinculin Sigma-Aldrich Cat# V9264; RRID: AB_10603627 mouse anti-Kindlin-2, clone 3A3 Millipore Cat# MAB2617; RRID: AB_10631873 rabbit anti-p34-Arc/ARPC2 Millipore Cat# 07-227 mNeonGreen-Trap Magnetic Agarose beads Chromotek Cat# ntma-20; RRID: AB_2827594 Chemicals, peptides, and recombinant proteins blasticidine S HCl Thermo R21001 Polyethylenimine Sigma 408727 Platinum SuperFi II PCR Master Mix Thermo Cat # 12368010 AF488-conjugated Phalloidin Thermo A12379 AF568-conjugated Phalloidin Thermo A12380 Bovine collagen, Type 1 Advanced Biomatrix Cat# 5133 rat-tail collagen Corning Cat# 354249 CK-666 Millipore Cat# 182515 purified Arp2/3 complex Cytoskeleton RP-01-PA QGel 920 CHT QGel 920 20% Paraformaldehyde Electron Microscopy Sciences 15713 Critical commercial assays Q5 site-directed PCR mutagenesis kit NEB E0554S DNeasy Blood and Tissue kit Qiagen Cat # 69504 QIAquick Gel Extraction Kit Qiagen Cat # 28704 Duolink® In Situ Detection Red kit Sigma DUO92008 Experimental models: Cell lines U2OS Gifted by Dick McIntosh (University of Colorado) RPE-1 ATCC CRL-4000; RRID: CVCL_4388 (Continued on next page) iScience 28, 112623, June 20, 2025 e1 .. Cell lines and culture conditions Human Osteosarcoma (U2OS; female) and human retinal pigment epithelial (RPE-1; female) cells were cultured in DMEM or RPMI media, respectively supplemented with 10% fetal bovine serum (Sigma; F0926-500ML) and maintained in a humidified incubator at 37◦C and 5% CO2.



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